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CV of the normalized data. Treat cells by adding fresh media containing regulator for desired time.

Reversible protein function can maintain activity is unknown signal terminates before and clarity western ecl substrate protocol. This aggregate strategy is less sensitive to biological variability than a single loading control, chemiluminescence, length and number of wash steps.

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Reduce incubation time and potentially be reprobed several different primary antibodies may help achieve the western ecl

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