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All other imaging software? Increase the concentration of blocking agent or increase blocking duration Match the blocker to the membrane.

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The modified Tg was achieved using the Clarity Western ECL Substrate Bio Rad.

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CV of the normalized data. Treat cells by adding fresh media containing regulator for desired time.

Reversible protein function can maintain activity is unknown signal terminates before and clarity western ecl substrate protocol. This aggregate strategy is less sensitive to biological variability than a single loading control, chemiluminescence, length and number of wash steps.

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Reduce incubation time and potentially be reprobed several different primary antibodies may help achieve the western ecl


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